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🧬 Molecular Biology Training Platform · v2.0 | Powered by Genoflow

PCR Learning
Laboratory

From zero to confident. Master Polymerase Chain Reaction through interactive theory, step-by-step calculations, virtual pipetting, and real gel interpretation.

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01

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Theory & Concepts

Understand what PCR is, why it matters, and the molecular mechanisms behind every step.

What is PCRCyclesComponents

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02

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Mix Calculations

Learn C₁V₁=C₂V₂ like a pro. Interactive calculator with step-by-step guided practice.

Dilution formulaInteractive calc

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03

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Virtual Lab Protocol

Hands-on pipetting simulation with tutorial arrows guiding you step by step.

Virtual pipettingTutorial arrows

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04

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PCR Programming

Design thermal cycling programs. Visualize temperature profiles and calculate Tm values.

Thermal cyclesTm calculator

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05

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Gel Electrophoresis

Interpret PCR results on interactive virtual gels. Diagnose failures like a scientist.

Band analysisCase studies

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Assessment & Certificate

Full knowledge test across all modules. Pass 80% to earn your proficiency certificate.

20 questionsCertificate

Select a module above to begin

🔬 Virtual Lab — PCR Setup

Follow the protocol steps on the right. Select a reagent → set volume → click Aspirate → click a tube → click Dispense.
Order: Water → Master Mix → Forward Primer → Reverse Primer → DNA Template (tubes 1-7 only).

📋 Protocol Checklist

💧Add 33 µL Water to all 8 tubes

🧪Add 25 µL Master Mix to all 8 tubes

➡️Add 1 µL Forward Primer to all 8 tubes

⬅️Add 1 µL Reverse Primer to all 8 tubes

🧬Add 1 µL DNA Template to tubes 1–7 only

📊 Tube Status

🧫 Reagent Shelf

🔊 Volume Control

33 µL

🧪 Tube Rack

🔧 Program Builder

🔥 Initial Denaturation 1×

Temp (°C)

Time (sec)

🔄 Cycling Block

cycles

🔥 Denaturation

Temp (°C)

Time (sec)

🧩 Annealing

Temp (°C)

Time (sec)

⚙️ Extension

Temp (°C)

Time (sec)

✅ Final Extension + Hold

Temp (°C)

Time (sec)

📈 Temperature Profile

Profile Preview

✓ Simulation Complete! Cycles run successfully.

📋 Program Summary

Step	Temp	Time	Cycles

🧬 Primer Tm Calculator

Primer Sequence (5'→3')

🧪 Your Lab Samples From Protocol Step

Your Name (for certificate)

Certificate of Achievement

PCR Techniques Proficiency

Molecular Biology — PCR Learning Laboratory

This certifies that

Student Name

has successfully demonstrated proficiency and mastery of Polymerase Chain Reaction techniques, completing all modules of the PCR Learning Laboratory program and passing the final assessment with distinction.

PCR Techniques Proficiency Program · GenoFlow Agency

20/20

Final Score

100%

Percentage

PASS

Status

Modules Completed

PCR Theory & Concepts

Mix Calculations

Virtual Lab Protocol

PCR Programming

Gel Electrophoresis

Assessment & Quiz

PCR LearningLaboratory

Theory & Concepts

Mix Calculations

Virtual Lab Protocol

PCR Programming

Gel Electrophoresis

Assessment & Certificate

🎉 Protocol Complete!

🔧 Program Builder

📈 Temperature Profile

PCR Learning
Laboratory